Intact Genomics COVID-19 or SARS-CoV-2 Coronavirus detection kit is used for in vitro detection of SARS-CoV-2 using the One Step RT-qPCR kit for SARSCoV-2 (COVID-19).  The coronavirus SARS-CoV-2 was announced as the etiological agent of cases of pneumonia outbreak. This Coronavirus detection kit allows efficient cDNA synthesis and qPCR in a single tube. This probe based one step RTqPCR 2x master mix contains Reverse Transcriptase, Taq DNA polymerase, RNase inhibitor, MgCl2, dNTPs, stabilizers and low ROX reference dye with proprietary buffer providing improved RT-qPCR efficiency, wider dynamic range, superior sensitivity and specificity. In addition, the kit contains CDC recommended primers/ probe sets. This kit can be used to detect SARS-CoV-2 in respiratory specimens such as sputum, nasopharyngeal, oropharyngeal aspirates, washes or swabs and tracheal aspirates.IgScript™ Reverse Transcriptase is a recombinant MMLV reverse transcriptase with reduced RNase H activity, increased thermostability and can produce cDNA from small amount of total RNA for real-time RT-qPCR analysis and other applications. Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase (1, 2) and a 5´→3´ exonuclease activity (3, 4). The amplification step features a high quality Taq DNA Polymerase which offers robust, reliable and better amplification.

One Step RT-qPCR kit includes:

• RT-qPCR 2x master mix
• CDC recommended primer/probe sets
  •  2019-nCoV_N1 (FAM)*
  •  2019-nCoV_N2 (FAM)*
  • RNase P (FAM)*
• COVID-19 positive control (PTC)

*Customization of Primer/Probe sets available upon request

 Benefits of One Step RT-qPCR kit

• Enhanced efficiency, specificity, and sensitivity
• Compatible with all real-time PCR instruments

 Storage Temperature

–20 °C

For research use only.  Not to be used for diagnostic purposes.

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Intact Genomics(ig)是大DNA片段克隆和宏基因组技术的世界领导者。我们有专门的科学家和最先进的设备，位于西线工业园，靠近拜耳作物科学总部、唐纳德·丹佛斯植物科学中心和圣路易斯的华盛顿大学。

我们提供高质量的生命科学产品，包括高质量的感受态细胞、强健的酶/蛋白质、大插入DNA克隆、操作和BAC文库相关服务，以帮助科学家探索微生物、植物和动物物种的基因组结构和功能。我们经验丰富的科学家团队提供卓越的个性化服务、快速的周转时间和业内最合理的价格。

我们还在宏基因组研究中应用BAC相关技术，为农业研究、天然产物酶发现和环境保护开发新的解决方案。在完整基因组公司，我们的专家是高分子量(HMW)基因组DNA制备、大DNA片段操作、无偏细菌人工染色体(BAC)文库构建、蛋白质表达和纯化领域的世界级领导者。我们持续关注基因组技术创新。