Intact Genomics FastAmp^® Plant Direct PCR kit is an easy and robust method to amplify DNA fragments from plant tissues with high specificity and high sensitivity without the need of complicated DNA purification steps.  The advanced formulation of this kit also allows fast PCR cycling conditions without compromising PCR sensitivity, specificity and yield. The FastAmp^® PCR kit is an ideal and powerful tool for high-throughput genotyping, DNA amplification, and plant genome analysis.

FastAmp^® Plant Direct PCR Kit is suitable for amplification of DNA directly from plant samples without purifying DNA. This kit is based on specially engineered Taq DNA polymerase, proprietary buffer system, dNTP, MgCl2 ,PCR facilitators and dye mix which makes it extremely robust and tolerant of plant PCR inhibitors such as complex polysaccharides, polyphenols and others. This kit has been tested with leaves and seeds from a wide variety of plant species. This PCR Kit includes a complete set of optimized reagents and detailed protocols making it an ideal choice for amplification of plant DNA without DNA purification.

Highlights

• Direct PCR- no need to purify DNA
• Specially engineered Taq DNA polymerase with highest sensitivity and specificity
• Extremely short PCR protocol times
• Master mix format with premixed gel loading dye to reduce cross-contamination and sample handlingerrors
• Optimized for both low and high GC templates

Applications

• Genotyping
• Transgene detection
• Knockout analysis
• Sequencing

 Product Includes

• FastAmp^® Plant Direct PCR master mix (2x)
• FastAmp^® Plant Direct PCR/Genotyping Solution

Storage Temperature–20ºC

Quality Control Assays

This PCR Kit has been tested with tissue from a wide variety of plant species, some of the results are included here.

Direct PCR analysis from various 1.2mm punched leaf tissue and scratch tissue using FastAmp^® Plant Direct PCR kit

Intact Genomics(ig)是大DNA片段克隆和宏基因组技术的世界领导者。我们有专门的科学家和最先进的设备，位于西线工业园，靠近拜耳作物科学总部、唐纳德·丹佛斯植物科学中心和圣路易斯的华盛顿大学。

我们提供高质量的生命科学产品，包括高质量的感受态细胞、强健的酶/蛋白质、大插入DNA克隆、操作和BAC文库相关服务，以帮助科学家探索微生物、植物和动物物种的基因组结构和功能。我们经验丰富的科学家团队提供卓越的个性化服务、快速的周转时间和业内最合理的价格。

我们还在宏基因组研究中应用BAC相关技术，为农业研究、天然产物酶发现和环境保护开发新的解决方案。在完整基因组公司，我们的专家是高分子量(HMW)基因组DNA制备、大DNA片段操作、无偏细菌人工染色体(BAC)文库构建、蛋白质表达和纯化领域的世界级领导者。我们持续关注基因组技术创新。