Intact Genomics (IG) i7^® High-Fidelity DNA Polymerase 2x master mix is ready to use premix which contains i7 high-fidelity DNA Polymerase, dNTPs, MgCl₂, PCR enhancers and stabilizers with optimized proprietary reaction buffer. i7 high fidelity DNA Polymerase is a genetically engineered, heat stable DNA polymerase which has 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. This enzyme has high fidelity, sensitivity and processivity with an error rate ~2.8×10²-fold lower than Taq DNA polymerase, and significantly lower than other proofreading enzymes in the marketplace (1). This 2x master mix product is supplied with 5x magic enhancer that enables efficient amplification of GC rich templates up to 84%.

Activity DataWe have tested i7^® high-fidelity 2x master mix with difficult templates for PCR amplification. Typical PCR results are shown below:

Applications

• Long and difficult template DNA amplification
• Cloning
• High-fidelity PCR
• Efficient for amplifying high GC content template DNA with magic enhancer.

 Product Includes

• i7^® high-fidelity DNA Polymerase 2x master mix
• 5x Magic Enhancer

Storage Temperature–20ºC

 Heat InactivationNo

 Storage Buffer50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25ºC3281 3283

Technical Support

Intact Genomics is committed to supporting the worldwide scientific research community by supplying the highest quality reagents. Each new lot of our products is tested to ensure they meet the quality standards and specifications designated for the product. Please follow the instructions carefully and contact us if additional assistance is needed. We appreciate your business and your feedback regarding the performance of our products in your applications.

Intact Genomics(ig)是大DNA片段克隆和宏基因组技术的世界领导者。我们有专门的科学家和最先进的设备，位于西线工业园，靠近拜耳作物科学总部、唐纳德·丹佛斯植物科学中心和圣路易斯的华盛顿大学。

我们提供高质量的生命科学产品，包括高质量的感受态细胞、强健的酶/蛋白质、大插入DNA克隆、操作和BAC文库相关服务，以帮助科学家探索微生物、植物和动物物种的基因组结构和功能。我们经验丰富的科学家团队提供卓越的个性化服务、快速的周转时间和业内最合理的价格。

我们还在宏基因组研究中应用BAC相关技术，为农业研究、天然产物酶发现和环境保护开发新的解决方案。在完整基因组公司，我们的专家是高分子量(HMW)基因组DNA制备、大DNA片段操作、无偏细菌人工染色体(BAC)文库构建、蛋白质表达和纯化领域的世界级领导者。我们持续关注基因组技术创新。