Intact Genomics (IG) i7^® High-Fidelity DNA Polymerase is a genetically engineered, heat stable DNA polymerase which has 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. This enzyme has the high-fidelity, sensitivity and processivity with an error rate ~2.8×10²-fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes in the marketplace (1). It is ideal for cloning and can be used for long (up to 20kb) or difficult amplicons. This product is supplied with the Intact Genomics proprietary 2.5x i7^® PCR reaction buffer containing MgCl₂ with a final (1x) concentration of 2 mM. This buffer allows for amplification of non GC rich templates and of GC rich templates up to 84%.

Protein PurityThe physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie® blue staining (see figure below).

Product SourceE. coli strain expressing genetically engineered i7^® High-Fidelity DNA Polymerase gene.

Comparison DataWe have tested this DNA Polymerase activity with λ DNA and other difficult templates for PCR amplification up to 20kb. Intact Genomics (IG) i7^® high-fidelity DNA Polymerase generates robust and high-quality PCR products in comparison with other high-fidelity DNA polymerases available in the marketplace (data shown below):

Applications

• Long and difficult template DNA amplification
• Cloning
• High-fidelity PCR
• Efficient for amplifying high GC content template DNA with magic enhancer

Product Includes

• i7^® High-Fidelity DNA Polymerase
• 2.5x i7^® PCR Buffer with Mg2+

Storage Buffer50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25ºC

 Storage Temperature–20ºC

Heat InactivationNo

Quality Control AssaysFree from detectable nuclease activities.

Unit DefinitionOne unit is defined as the amount of enzyme thatincorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72º C.3254 3255

Intact Genomics(ig)是大DNA片段克隆和宏基因组技术的世界领导者。我们有专门的科学家和最先进的设备，位于西线工业园，靠近拜耳作物科学总部、唐纳德·丹佛斯植物科学中心和圣路易斯的华盛顿大学。

我们提供高质量的生命科学产品，包括高质量的感受态细胞、强健的酶/蛋白质、大插入DNA克隆、操作和BAC文库相关服务，以帮助科学家探索微生物、植物和动物物种的基因组结构和功能。我们经验丰富的科学家团队提供卓越的个性化服务、快速的周转时间和业内最合理的价格。

我们还在宏基因组研究中应用BAC相关技术，为农业研究、天然产物酶发现和环境保护开发新的解决方案。在完整基因组公司，我们的专家是高分子量(HMW)基因组DNA制备、大DNA片段操作、无偏细菌人工染色体(BAC)文库构建、蛋白质表达和纯化领域的世界级领导者。我们持续关注基因组技术创新。