Pfu DNA Polymerase 2x master mix is ready to use premix which contains Pfu DNA Polymerase, dNTPs, MgCl₂ and stabilizers with optimized reaction buffer. It has been optimized for routine PCR applications. Pfu DNA Polymerase is a heat stable DNA polymerase which has 5´→ 3´ DNA polymerase and 3´→ 5´ exonuclease (proofreading) activities. Pfu DNA polymerase retains the high fidelity, sensitivity and processivity with an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the error rates of most other proofreading enzymes or DNA polymerase mixtures (1). Pfu 2x Master Mix product is supplied with the unique Intact Genomics 5x Magic Enhancer that enables efficient amplification of GC rich templates up to 84%.

Product SourceE. coli strain expressing a Pfu DNA Polymerase gene from Pyrococcus furiosus.

Pfu DNA Polymerase Comparison Data

We repeatedly compare our Pfu Polymerase side-by-side with a leading competitor for PCR assay. Our enzyme is better than the competitor.  A typical gel picture is shown below:

Applications

• Routine PCR
• Primer extension
• Colony PCR
• Genotyping
• Efficient for amplifying high GC content template DNA with Magic Enhancer

 Product Includes

1. Pfu 2x master mix
2. 5x Magic Enhancer

 Storage Temperature–20 °C

1x Master Mix Composition

10 mM Tris-HCl pH 9.0, 50 mM KCl, 1.5 mM MgCl_2, 0.2 mM dNTPs, 5% Glycerol, 0.08% Igepal CA 630, 0.05% Tween-20, 100 Units/ml Pfu DNA Polymerase.3325 3326

Intact Genomics(ig)是大DNA片段克隆和宏基因组技术的世界领导者。我们有专门的科学家和最先进的设备，位于西线工业园，靠近拜耳作物科学总部、唐纳德·丹佛斯植物科学中心和圣路易斯的华盛顿大学。

我们提供高质量的生命科学产品，包括高质量的感受态细胞、强健的酶/蛋白质、大插入DNA克隆、操作和BAC文库相关服务，以帮助科学家探索微生物、植物和动物物种的基因组结构和功能。我们经验丰富的科学家团队提供卓越的个性化服务、快速的周转时间和业内最合理的价格。

我们还在宏基因组研究中应用BAC相关技术，为农业研究、天然产物酶发现和环境保护开发新的解决方案。在完整基因组公司，我们的专家是高分子量(HMW)基因组DNA制备、大DNA片段操作、无偏细菌人工染色体(BAC)文库构建、蛋白质表达和纯化领域的世界级领导者。我们持续关注基因组技术创新。