Hot start Taq DNA Polymerase 2x master mix is ready to use premix which contains hot start Taq DNA Polymerase, dNTPs, MgCl₂ and stabilizers with optimized reaction buffer. Intact Genomics Hot Start Taq 2x Master Mix has been optimized for routine PCR applications. Hot start Taq is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). Hot Start Taq DNA Polymerase is chemically modified that leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. Hot start PCR reduces non-specific amplification during setup stages of the reaction and helps increase PCR specificity and sensitivity. This product is supplied with the unique Intact Genomics 5X Magic Enhancer that enables efficient amplification of GC rich templates up to 84%.

Applications

• Routine PCR and RT-PCR
• Primer extension
• Colony PCR
• Genotyping
• Efficient for amplifying high GC content template DNA with Magic Enhancer.

 Product Includes

1. Hot start Taq 2x master mix
2. 5X Magic Enhancer

1x Master Mix Composition

10 mM Tris-HCl pH 9.0, 50 mM KCl, 1.5 mM MgCl_2, 0.2 mM dNTPs, 5% Glycerol, 0.08% Igepal CA 630, 0.05% Tween-20, 100 Units/ml Hot start Taq Polymerase.3295 3296 3297 3299

Intact Genomics(ig)是大DNA片段克隆和宏基因组技术的世界领导者。我们有专门的科学家和最先进的设备，位于西线工业园，靠近拜耳作物科学总部、唐纳德·丹佛斯植物科学中心和圣路易斯的华盛顿大学。

我们提供高质量的生命科学产品，包括高质量的感受态细胞、强健的酶/蛋白质、大插入DNA克隆、操作和BAC文库相关服务，以帮助科学家探索微生物、植物和动物物种的基因组结构和功能。我们经验丰富的科学家团队提供卓越的个性化服务、快速的周转时间和业内最合理的价格。

我们还在宏基因组研究中应用BAC相关技术，为农业研究、天然产物酶发现和环境保护开发新的解决方案。在完整基因组公司，我们的专家是高分子量(HMW)基因组DNA制备、大DNA片段操作、无偏细菌人工染色体(BAC)文库构建、蛋白质表达和纯化领域的世界级领导者。我们持续关注基因组技术创新。